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wrote...
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5 years ago
Activity 2: Exploring Amylase Substrate Specificity
1. Describe why the results in Tube 1 and Tube 2 are the same.

2. Describe the result in tube 3.

Activity 3: Assesing Pepsin Digestion of Protein
1. What do you think would hapen if you reduced the incubation time to 30 minutes for tube 5?

Activity 4: Assessing Lipase Digestion of Fat
1. Explain why you can't fully test the lipase activity in the tube 5.

2.Which tube had the highest lipase activity?

3. Explain why pancreatic lipase would be active in both the mouth and the intestine.

4. Describe the process of bile emulsification of lipids and how it improves lipase activity.

Any help will be great! Thanks!
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5 years ago
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1. Explain why you can't fully test the lipase activity in the tube 5.

Measurement of lipase activity uses a decrease in pH. Since the pH in Tube #5 is already very low, it is difficult to tell if fatty acids are released.

Test tube #1 should have the highest activity because the pH is closest to the pH of the small intestine. Note: Some students might choose pH 9.0 but the intestine is closer to 8.0 and the enzyme is not activity at such high alkalinity.
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wrote...
5 years ago
Thanks for the help!
wrote...
5 years ago
Thanks for the help!

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